Cell Line Origin
Human Caucasian breast adenocarcinoma
Cell Line Description
Isolated from pleural effusions of a breast cancer patient.
Application
MDA-MB-231 has been used to study:
- the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies
- the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system
- the transendothelial migration rates of the cells under different shear stress levels
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18
Culture Medium
L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
biological source: human breast. growth mode: Adherent. karyotype: Modal no.′. s 62 and 64, near triploid. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181946
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92020424-1VL
