Cell Line Origin
Canine Cocker Spaniel kidney
Cell Line Description
Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).
Application
Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: dog kidney. growth mode: Adherent. karyotype: 2n : 78. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41104132
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85011435-1VL
