Endoscopic ligators

Application Suitable for: Ligation of blunt ended or cohesive DNA fragments Ligation of cloning vector and restriction insert fragments Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids Couple RNA single strands by bridging

DNA Ligation

General description Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions. Sticky-end ligations are more efficient than blunt-end ligations: these can be facilitated with the addition of PEG.

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. &nbspLigation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which

Floor Grade, Single Use Instrument

One unit is defined as the amount of enzyme required to ligate 50% of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12μM, 300μg/ml) in a total reaction volume of 20μl in 30 minutes at 16℃.

One unit is defined as the amount of enzyme required to ligate 50% of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12μM, 300μg/ml) in a total reaction volume of 20μl in 30 minutes at 16℃.

One unit is defined as the amount of enzyme required to ligate 50% of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12μM, 300μg/ml) in a total reaction volume of 20μl in 30 minutes at 16℃.

ENDURO GEL SPACER 2mm

EPPENDORF(TM) DNA LOBIND TUBES

EPPENDORF(TM) DNA LOBIND TUBES

LIGAMAX 5 Endoscopic Multiple Clip Applier, 5mm, 15 Medium/Large Clips, 3/bx (Continental US Only)