General description
A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA104, 6235.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 272.39 (free base basis). Empirical Formula: C17H24N2O · xHCl. Quality Level: 100. Assay: ≥. 97% (HPLC). form: solid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. color: pale yellow. solubility: water: 1 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51285103
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 444805-10MG
