MES monohydrate (C09-0968-339)

General DescriptionMES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in molecular biology.ApplicationMES Monohydrate has been used: In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer As a component of Murashige and Skoog basal salt media In the preparation of total ionic strength adjustment buffer (TISAB)Other NotesSolubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months. Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown. Specifications and Purity: Ultrapure BioReagent, for molecular biology, ≥99.5% (T). Molecular Formula: C6H13NO4S·H2O. Molecular Weight: 213.25. EC Number: 224-632-3. PubChem CID: 16218417. Isomeric SMILES: C1COCCN1CCS(=O)(=O)O.O. Beilstein Registry Number: 6350956. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/M432121-SCI_edfd0d59bce3bc31093d9d9e3ae701d1.pdf.