Cell Line Origin
Human uterus sarcoma
Cell Line Description
The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
Application
Tumour toxicity assays and multi-drug-resistant studies.
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37°C. Tap side of flask to dislodge cells.
Other Notes
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biological source: human uterus. growth mode: Adherent. karyotype: Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3. morphology: Epithelial. products: High levels of Mdr1 mRNA and P-glycoprotein. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51111859
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95051031-1VL
