Skip to main content

MSC FREEZING MEDIUM 50 ML

Catalog No.
C005B-315392
Mfr. No.
SCM016
Mfr. Name
Sigma-Aldrich
Qty/UOM
50
UOM
ML
Price: $315.08
List Price: $350.09

Application 1. Thaw cell culture freezing medium completely and mix well by gently swirling bottle.

Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. Contact Us

Adding to cart… The item has been added

Application

1. Thaw cell culture freezing medium completely and mix well by gently swirling bottle. Keep freezing medium on ice during use.
2. Cells to be frozen should be in late log phase growth.
3. Monolayers will need to be dissociated. After dissociation, cells are resuspended in Mesenchymal Stem Cell Expansion Medium (Cat. No. SCM015) and counted to determine viability and number.
4. Centrifuge cells at 300 x g for 3 min. Remove the medium above the pellet.
5. Resuspend the cells in Mesenchymall Stem Cell Freezing Medium at a concentration of ~4 x 106 cells/mL. Freeze 1 mL of cells/vial. After the cells have been resuspended and aliquoted into appropriate cryogenic storage vials, they can be placed in a freezing container and the normal freeze down procedure should be started within five minutes.
6. Cells must be stored at or below -80°C. For long term storage the cells should be stored in ultra-low temperature freezer (-150°C), or in liquid nitrogen (-196°C).
7. Thawing of cryopreserved cells should be as follows:
a. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
b. Thaw cells quickly in a 37°C water bath. Important: Do not vortex the cells.
c. Sterilize vial by rinsing with 70% ethanol.
d. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
e. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
f. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
g. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
h. Decant as much of the supernatant as possible
i. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C).
j. Plate the cell mixture onto a 10-cm tissue culture plate.
k. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
l. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
m. When the cells are approximately 80% confluent, they can be dissociated with Accutase® (Cat. No. SCR005) and passaged or alternatively frozen for later use.

Physical form

Serum-containing formulation. Contains 10% DMSO.

Storage and Stability

Store at -20°C.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

More Information: https://cenmed.com/shipping-returns

UPC:
51294211
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
SCM016
Temperature Control Device:
Yes

Cenmed Satisfaction Guarantee
Cenmed Satisfaction Guarantee

At Cenmed, your confidence and satisfaction are paramount. We guarantee the quality and reliability of our extensive range of clinical and laboratory supplies. If you're not completely satisfied with your purchase, we offer a straightforward return process and dedicated support to resolve your concerns promptly. Our commitment ensures that you can order with confidence, knowing that Cenmed is dedicated to superior service and customer satisfaction. Trust us to meet your needs with every order, backed by our promise of excellence. Learn more in Help & FAQs.

Testimonial
"Cenmed provides me access to the same products/services normally reserved for much larger labs than mine. I was presently surprised by their product offering."

LAB DIRECTOR

Testimonial
"We utilized Cenmed's capabilities for a variety of projects around the world. They are a valued partner and supplier."

PHARMACEUTICAL SUPPLY CHAIN LEADER

Testimonial
"The reps are very good at finding products for customers in this period of supply chain issues."

SCOTT BEHMAN

Testimonial
"Your customer service has been excellent and makes me excited about purchasing with Cenmed in the future!!"

PROCUREMENT + BILLING COORDINATOR AT PHARMA.