InformationMethacycline HCl Methacycline HCl (Rondomycin) is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.In vitroMethacycline/xa0inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline inhibits TGF-β1-induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.In vivoMethacycline/xa0(100 mg/kg, i.p.) delivered daily beginning 10 days after intratracheal bleomycin improves survival at Day 17. Methacycline attenuates Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA. Methacycline/xa0does not attenuate inflammatory cell accumulation or alter TGF-β1-responsive genes in alveolar macrophages./xa0Cell Datacell lines：Concentrations：Incubation Time：Powder Purity:≥99%. Specification: 10mM in Water Molecular Formula: C22H22N2O8·HCl Molecular Weight: 478.88
- UPC:
- 12352002
- Condition:
- New
- Availability:
- 2 weeks
- Weight:
- 0.85 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M407975-1ml
- CAS:
- 3963-95-9
- Product Size:
- 1ml
