General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Specificity
Antibody recognizes Tetrahymena acetyl Histone H3
This sequence is highly conserved and predicted to have broad species crossreactivity.
Immunogen
KLH-conjugated peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC) where K* is acetylated
Application
This Milli-Mark Anti-acetyl-Histone H3-PE Antibody is validated for use in FC for the detection of acetyl-Histone H3.
Quality
Evaluated by flow cytometry using HeLa cells
Target description
15.5 kDa Calculated
Physical form
Purified rabbit polyclonal IgG conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41181551
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- FCABS325PE
