General description
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Specificity
Reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
Immunogen
4-8 cell stage mouse embryos
Application
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Milli-Mark Anti-SSEA3 Alexa 488 Antibody, clone MC-631 is validated for use in FC for the detection of SSEA3 Alexa 488.
Quality
Evaluated by flow cytometry using 2102 Ep cells.
Physical form
Purified mouse monoclonal IgM conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
2102 Ep Cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Unconjugated antibody Cat. No. MAB4303-I is known to react with mouse.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- FCMAB141A4
