Mini Columns

Gel filtration is a commonly used technique for buffer exchange and desalting, removal of small unincorporated labeled nucleotide products from DNA, riboprobe cleanup, and dideoxy terminator removal and protein purification between 6,000 and 40,000 molecular weight. IBI offers pre-made gel filtration columns with either G-25 or G-50 Sephadex® saturated with either STE or TE buffer. All columns are RNase- and DNase-free. The Select-D Sephadex® G-25 columns are intended for purification of DNA, including reaction mixtures, desalting, recovering DNA fragment (>12mer) unincorporated radio labeled deoxynucleotide triphosphates (dNTPs) from small volume 5’-end-labeling reactions, primer-dimer, fill-in labeling reactions, and enzymes that may inhibit subsequent applications. The Select-D G-25 are pre-packed spin columns for rapid purification of oligoribonucleotides and RNA. These columns are also RNase- and DNase-free and are recommended for 12mer up to 72bp. The Select-D G-50 are pre-packed spin columns for rapid purification of oligoribonucleotides and RNA. The columns are RNase- and DNase-free and recommended for purification of DNA or RNA greater than 72bp in length. All MINI and MIDI spin column kits come complete with 2ml collection tubes, while the MAXI Select-D and Nu-Clean spin column kits come complete with 1ml collection tubes IBI Spin Columns are available in three sizes; MINI (20µl), MIDI (50µl), and MAXI(100µl) These columns are pre-hydrated with either TE or STE buffer for ease of use and extended shelf life