Mini High-Speed Plasmid Kit-300 Preps

Fast and economical plasmid minipreps IBI’s High-Speed MINI Plasmid Kit is designed for rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNase treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate, binds to the glass-fiber matrix in the spin column. The contaminants are washed off with an ethanol based wash buffer, and the purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does NOT require DNA phenol extraction or alcohol precipitation. Typical yields are 20-30mg for high-copy numbered plasmid or 3-10mg for low-copy numbered plasmid. The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, or sequencing reactions. This entire procedure can be completed in under 30 minutes.