General description
8 x 20mm
Features and Benefits
MiniChrom Column Fractogel® COO (M), 1 ml enables:
- Good resolution between monomers and aggregates
- Efficient aggregate and HCP removal combined with high yield
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Analysis Note
Appearance: passes test
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41115716
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 1250620001