Mitsuhashi and Maramorosch Insect Medium, w/o Sodium Bicarbonate, Powder, 20 L

Mitsuhashi and Maramorosch Insect Medium is specially used for the growth and propogation of mosquito cell lines especially Aedes aegypticus . It was originally developed to grow cells derived from Leafhopper. Subsequently, it has been used to culture cells derived from a number of insect species. IM002 is Mitsuhashi amd Maramorosch Insect Medium. It needs to be supplemented with 5-20 % fetal bovine serum. Lactalbumin hydrolysate serves as a source of free amino acid, whereas yeast extract serves as a source of vitamins. When supplemented with fetal bovine serum, this medium is most commonly used to culture cells derived from a number of mosquitoes. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. Appearance of Powder: Off-white to creamish white, homogenous powder Solubility: Clear solution at 23.1gms/L pH without Sodium Bicarbonate: 6.20 -6.80 pH with Sodium Bicarbonate: 6.30 -6.90 Osmolality without Sodium Bicarbonate: 300.00 -340.00 Osmolality with Sodium Bicarbonate: 310.00 -350.00 Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures. Endotoxin Content: NMT 10EU/ml Storage and Shelf-life: 1. All the powdered media and prepared liquid culture media should be stored at 2-8°C.Use before the expiry date. Inspite of above recommended storage condition, certain powdered medium may show some signs of deterioration /degradation in certain instances. This can be indicated by change in colour, change in appearance and presence of particulate matter and haziness after dissolution. 2. pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For example, in large bottles, such as Roux bottles pH tends to rise perceptibly as significant volume of carbon dioxide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration, surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dioxide. 3. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions. Shelf life of the medium will depend on the nature of supplement added to the medium.