MMP-9-LIPOCALIN-TIMP-1 COMPL 1PC X 5UG

General description

A quenched fluorescence substrate for MMP-13 (collagenase-3; k_cat/K_m = 1.09 x 10⁶ M^-1s^-1). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).

A quenched fluorescence substrate for MMP-13 (collagenase-3; k_cat/K_m = 1.09 x 10⁶ M^-1s^-1; excitation maximum: ~325 nm; emission maximum: ~393 nm). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).

Biochem/physiol Actions

Primary Target
A quenched fluorescence substrate for MMP-13 (collagenase-3)

kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)

Warning

Toxicity: Standard Handling (A)

Sequence

MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Knauper, V., et al. 1996. J. Biol. Chem. 271, 1544.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany