Modified Ova Mrna: Modified Ovalbumin Encoding Mrna 20?g

OVA mRNA encodes for OVA protein, a commonly used antigen for immunization and biochemical studies and also an established model allergen for airway hyper-responsiveness. This modified mRNAs reduce this effect but last longer in the organism due to the stabilization and protection. They are capped (Cap 0) and polyadenylated mRNA optimized for mammalian systems with modified nucleotids to reduce immune stimulation. mRNA Vaccines offer several advantages over traditional DNA and viral-based approaches: Express the desired antigen and act as an adjuvant. Prevent risk of genomic integration and no inactivated viruses or pathogens are needed. No need of nuclear uptake - protein expression directly in cytoplasm Perfect for transfecting slowing or non-dividing cells such as Antigen Presenting cells (DCs) Faster protein expression than DNA transfection Protein expression in a total promoter-independent manner Transient transfection: mRNA based expression of proteins sustains for a limited time β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. it is a common marker gene used to assess transfection efficiency. They are capped (Cap 0) and polyadenylated mRNA optimized for mammalian systems with modified nucleotids to reduce immune stimulation. mRNA Vaccines offer several advantages over traditional DNA and viral-based approaches: Express the desired antigen and act as an adjuvant. Prevent risk of genomic integration and no inactivated viruses or pathogens are needed. No need of nuclear uptake - protein expression directly in cytoplasm Perfect for transfecting slowing or non-dividing cells such as Antigen Presenting cells (DCs) Faster protein expression than DNA transfection Protein expression in a total promoter-independent manner Transient transfection: mRNA based expression of proteins sustains for a limited time