Cell Line Origin
Human B lymphocyte, EBV transformed
Cell Line Description
Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions in the human immunoglobulin heavy chain constant region locus: deletion I (G1-EP1-A1-GP-G2-G4) and deletion II (EP1-A1-GP). Gm-Am phenotype: Gmb:-;A2m2/Gmg;za;.A2m2. The absence of G2m allotypes due to the deletion of the IGHG2 gene.
Application
Molecular and immunological analysis of gene deletions
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93093002-1VL
