Molecular Biology

CRISPRi is a potent tool for modulating gene expression. This technology uses dCas9 to act as a synthetic transcription factors by recruiting endogenous transcription repressor complexes to gene promoters and enhancers, resulting in down regulated

The transcriptional activator VP64 fused to nuclease-dead SpCas9 (dCas9), forms a ribonucleoprotein complex (RNP) with the CRISPR guide RNA (gRNA). The stem- and tetra-loop sequences in the gRNA scaffold have been modified into minimal hairpin RNA

The transcriptional activator VP64 fused to nuclease-dead SpCas9 (dCas9) forms a ribonucleoprotein complex (RNP) with the CRISPR guide RNA (gRNA). The stem- and tetra-loop sequences in the gRNA scaffold have been modified into minimal hairpin RNA

Cell Line Origin Mouse / Human hybrid, SV-40 transformed Cell Line Description The cell line CL21 has been established by hybridisation of C57 BL mouse peritoneal macrophages with SV40 transformed human skin fibroblasts. The human cells were

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free. The HumanKine Thermostable bFGF is engineered with

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free. The HumanKine Thermostable bFGF is engineered with

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free. The HumanKine Thermostable bFGF is engineered with

Packaging SKU-pack size numbers ending with a 3" are SmartChemicals with an RFID tag on the label for seamless data transfer to the instrument, e.g."

Packaging SKU-pack size numbers ending with a 3" are SmartChemicals with an RFID tag on the label for seamless data transfer to the instrument, e.g."

0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.

0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches