Proteins & Enzymes

A specific substrate for pancreatic elastase (K m = 100 &#181M K cat /K m = 35,000 s -1 M -1 for rat pancreatic elastase K m = 30 &#181M K cat /K m = 351,000 s -1 M -1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at

GranuCult &#174 plus EMB Agar acc. HOLT-HARRIS and TEAGUE is a low selective and differential medium containing lactose and sucrose and two indicator dyes, Eosin and Methylene Blue.

Application A selective medium for the differentiation of lactose fermenting and lactose non-fermenting intestinal organisms. Preparation Note Suspend 41 g in 1 litre of distilled water.

Note: 1 mU = 1 milliunit. Recombinant, Streptococcus pneumoniae Endo-&#945-N-acetylgalactosaminidase expressed in E.

Application Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry.

Note: 1 mU = 1 milliunit. Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E.

Note: 1 mU = 1 milliunit. Recombinant, Elizabethkingia meningosepticum endoglycosidase F2 expressed in E.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli .

Endoglycosidase H or endo-&#946- N -acetylglucosaminidase H&nbspis an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.

Note: 1 mU = 1 milliunit. Recombinant, streptomyces plicatus endoglycosidase H expressed in E.

Native endopeptidase Lys-C from Achromobacter lyticus . Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues.

The wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly