DNA Ligases

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles.

Application Suitable for: Ligation of blunt ended or cohesive DNA fragments Ligation of cloning vector and restriction insert fragments Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids Couple RNA single strands by bridging

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3&#8242-hydroxyl- and 5&#8242-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.

Eppendorf Tubes are the perfect option for working with medium-sized sample volumes! &ltUL&gt&ltLI&gtMaximum safety and stability for centrifugation up to 25,000 × g&lt/LI&gt&ltLI&gtConical bottoms&lt/LI&gt&ltLI&gtEasier processing of

The purified FnCas9 protein (~190 kDa) is derived from the type II CRISPR/Cas system from the bacterium Francisella novicida and has been engineered to function using three molecular components: the FnCas9 protein, a crRNA of user-defined sequence,

The purified FnCas9 protein (~190 kDa) is derived from the type II CRISPR/Cas system from the bacterium Francisella novicida and has been engineered to function using three molecular components: the FnCas9 protein, a crRNA of user-defined sequence,

Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system.

CRISPRi is a potent tool for modulating gene expression. This technology uses dCas9 to act as a synthetic transcription factors by recruiting endogenous transcription repressor complexes to gene promoters and enhancers, resulting in down regulated

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera -derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.

PrEST Antigen AARS2 alanyl-tRNA synthetase 2, mitochondrial,

PrEST Antigen AARS2 alanyl-tRNA synthetase 2, mitochondrial,

PrEST Antigen C8orf88 chromosome 8 open reading frame 88, 10