Mono-PolyT Resolving Medium 100mL

Mono-Poly Resolving Medium is a sterile preparation for in vitro isolation of mononuclear and polymorphonuclear leukocytes from whole blood. It separates mononuclear and polymorphonuclear leukocytes from whole human blood in two distinct bands. Mono-Poly™ is highly versatile and can be used for a variety of research and clinical applications such as histocompatibility testing, lymphocyte studies, in vitro cell mediated immunity assays, or any procedure requiring pure white blood cell preparation. Only fresh blood should be used to ensure good separation and high viability of isolated cells. However, acceptable separation can be obtained for up to 3 hours after blood collection. Functionality is assessed by using the medium to separate both mononuclear and polymorphonuclear leukocyte fractions free of erythrocyte contamination from human whole blood.