Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse

General description

Monoclonal Anti-Acetyl & Phospho-Histone H3 (Ac-Lys⁹, pSer¹⁰) (mouse IgG2a isotype) is derived from the APH3-64 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic, acetylated and phosphorylated histone H3 peptide. Histone H3 is a component of the histone octamer of the nucleosome DNA complex. It possesses a lysine-rich N-terminal tail region.

Specificity

Monoclonal Anti-Acetyl & Phospho-Histone H3 (Ac-Lys, pSer) specifically recognizes human histone H3 only when simultaneously acetylated on Lys and phosphorylated at Ser.

Immunogen

synthetic, acetylated and phosphorylated histone H3 peptide (amino acids 7-20, Ac-Lys⁹, pSer¹⁰) corresponding to the N-terminus of human histone H3. The sequence is identical in many species.

Application

Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys⁹, pSer¹⁰) antibody produced in mouse has been used in:

• chromatin immunoprecipitation
• antibody microarray
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation

Biochem/physiol Actions

Acetylation of histones on lysine residues within the N-terminal domain by histone acetyl-transferase (HATs) including histone acetyltransferase (Gcn5p), p300/CREB-binding protein-associated factor (P/CAF), E1A binding protein p300/CREB-binding protein (p300/CBP), and transcription initiation factor TFIID 250 kDa subunit (TAFII250) is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure making it more accessible to transcription complexes. In most species, histone H3 is primarily acetylated at lysines 9, 14, 18, and 23. In some organisms, acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly. Phosphorylation of histone H3 on Ser10 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at serine is implicated with the induction of immediate-early oncogenes like c-jun, c-fos, and c-myc. Protein kinase A (PKA), 90 kDa ribosomal S6 kinase-2 (Rsk-2), and MAP- and Stress-activated kinase 1 (Msk-1) are necessary for histone H3 phosphorylation. The ERK and p38 pathways activate Msk-1 to phosphorylate histone H3. Monoclonal antibodies to acetylated and phosphorylated histone H3 are an important tool for studying chromatin remodeling and gene regulation.

Physical form

Solution 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.