MOUSE MONOCLONAL ANTI-A-ACTININ (SARCOMERIC) CLONE EA-53 (C005B-239285)

General description

α-Actinin (ACTN2) is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. ACTN2 is located on human chromosome lq42-q43. α-actinin has rod shaped N-terminal domain.
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.
The antibody is specific for a-skeletal and a-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. α-actinin is detected predominantly in dense bodies and plaques which are characteristic of that tissue. Immunofluorescent labeling of a large variety of cells with anti a -actinin reveals an extensive association of the proteins with the actin containing stress fibers and, in particular, with their membrane-bound termini.
Monoclonal Anti-α-Actinin (Sarcomeric) (mouse IgG1 isotype) is derived from the EA-53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit skeletal a-actinin.1 The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-α-Actinin (Sarcomeric) may be used for the localization of sarcomeric α-actinin using various immunochemical assays such as ELISA, dot blot, immunoblot, immunohistochemistry, and immunocytochemistry. The antibody is useful in the immunolocalization of α-actinin in normal and neoplastic cultured cells and tissues, and for studies on the state of sarcomeric muscle organization, in normal and pathological situations.

Specificity

The antibody is specific for a-skeletal and α-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).

Immunogen

Rabbit skeletal α-actinin

Application

Cardiac myocytes were stained with monoclonal mouse anti-actinin to visualize the Z-lines after fixation in pre-cooled methanol/acetone mix (1:1) for 10 minutes at 4 °C.

Mouse monoclonal anti-α-actinin (sarcomeric) antibody is suitable for immunohistochemistry (1:800), ELISA, immunofluorescence and western blot (1:2,500) applications.

Biochem/physiol Actions

α-actinin functions as an actin crosslinker and promotes cell migration. Mutations in α-actinin gene locus is observed in patients with heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Physical form

Supplied as ascites fluid with 15 mM sodium azide as a preservative

Storage and Stability

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. Quality Level: 200. conjugate: unconjugated. antibody form: ascites fluid. antibody product type: primary antibodies. clone: EA-53, monoclonal. mol wt: antigen 100 . kDa. contains: 15 . mM sodium azide. species reactivity: mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard. technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle, indirect ELISA: suitable using human and animal muscle tissue, indirect immunofluorescence: suitable using human and animal cultured muscle cells, western blot: 1:2,500 using rat leg muscle. isotype: IgG1. UniProt accession no.: P35609. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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