Monoclonal Anti-alpha-Adaptin antibody produced in mouse (C15-1315-174)

General description

Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.

Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.

The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.

Immunogen

AP-2 adaptor polypeptides from bovine brain.

Application

Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.

Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.

Mouse monoclonal anti-α-adaptin antibody has been used:

• as a positive control in immunoblotting assays
• as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines

Biochem/physiol Actions

Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. conjugate: unconjugated. antibody form: ascites fluid. antibody product type: primary antibodies. clone: 100/2, monoclonal. contains: 15 . mM sodium azide. species reactivity: bovine, human, rat. technique(s): western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex. isotype: IgG2a. UniProt accession no.: O95782. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... AP2A1(160)
rat ... Ap2a1(308578). Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.