MONOCLONAL ANTI-ATRX ANTIBODY PRODUCED IN MOUSE (C15-1318-539)

General description

ATRX (α-thalassemia/mental retardation syndrome X-linked) is encoded by the gene mapped to human chromosome Xq21.1. The encoded protein belongs to SWI/SNF (SWItch/sucrose non-fermentable) family of chromatin remodeling proteins.

Immunogen

alpha thalassemia/mental retardation syndrome X-linked, recombinant protein epitope signature tag (PrEST)

Sequence
AAWAEYEAEKKGLTMRFNIPTGTNLPPVSFNSQTPYIPFNLGALSAMSNQQLEDLINQGREKVVEATNSVTAVRIQPLEDIISAVWKENMNLSEAQVQALALSRQASQELDVKRREAIYNDVLTKQQMLISCVQRILMNRR

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

ATRX (α-thalassemia/mental retardation syndrome X-linked) is involved in the H3.3-ATRX-DAXX chromatin-remodeling pathway. It plays a vital role in assimilation of the histone variant H3.3 at pericentric heterochromatin and at telomeres, as well as at several transcription factor binding sites. Mutation in the gene has been observed in various types of gliomas. Thus, this protein can be considered as a potential biomarker for gliomas. In addition, aberrations in the gene expression leads to characteristic developmental abnormalities, such as severe mental retardation, facial dysmorphism, urogenital abnormalities and α-thalassemia.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST74299.

Physical form

Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: mouse. Quality Level: 100. conjugate: unconjugated. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: CL0537, monoclonal. product line: Prestige Antibodies^®. Powered by Atlas Antibodies. form: buffered aqueous glycerol solution. species reactivity: human. packaging: antibody small pack of 25 . μ. L. enhanced validation: RNAi knockdown
Learn more about Antibody Enhanced Validation. technique(s): immunoblotting: 1 . μ. g/mL, immunofluorescence: 2-10 . μ. g/mL, immunohistochemistry: 1:200- 1:500. isotype: IgG1. Ensembl | human accession no.: ENSG00000085224. UniProt accession no.: P46100. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... ATRX(546). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.