Monoclonal Anti-CD8 antibody produced in mouse

General description

Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human thymocytes followed by peripheral blood T cells. CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12.

Specificity

Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567

Immunogen

human thymocytes followed by peripheral blood T cells.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)

Monoclonal Anti-CD8 antibody produced in mouse is has been used for the following:

• enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids
• identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues
• analysis of cell mediated cytotoxicity
• studies of immunoregulation in health and disease
• investigation of natural killer (NK) cells
• complement mediated cytolysis of CD8 expressing cells

Monoclonal Anti-CD8 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.

Biochem/physiol Actions

CD8 might serve as a marker for neoplastic diseases and acute myeloid leukemia.

CD8a (Cluster of Differentiation 8a) is a human gene. CD8 binding to class I MHC and the co-receptor′s during the process of cytotoxic T-cell activation. T cells expresses T cell receptor (TCR) complexes which lacks CD3 delta, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 delta couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8(+) T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).

Target description

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56^lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.