General description

Monoclonal Anti-Dynein (Heavy Chain) (mouse IgG2a isotype) is derived from the 440.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dynein axonemal heavy chain 1 (DNAH1) encodes for an inner dynein heavy chain. This gene is mapped to the human chromosome location 3p21.1. DNAH1 is expressed in the testis and other ciliated cells. Dyneins are composed of two or three distinct heavy chains, three intermediate chains and at least four light chains.

Immunogen

cytoplasmic dynein from chicken embryo brain.

Application

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse has been used in:

indirect immunofluorescence at 1:50 dilution
immunocytochemistry
enzyme-linked immunosorbent assay (ELISA)
immunoblotting
dot blot

Biochem/physiol Actions

Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport. It also regulates the endocytic pathway and the organization of the Golgi apparatus. Mutations in dynein axonemal heavy chain 1 (DNAH1) are associated with primary ciliary dyskinesia and multiple morphological anomalies of the flagella (MMAF) that leads to asthenozoospermia and male infertility.

Dynein is a motor protein that regulates the movement of organelles to the â€˜minusâ€² end of microtubules. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement

Physical form

Suppied as ascites fluid with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.