General description
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
- in direct tissue blot immunoassay of sweet orange petioles samples
- in screening internalization of delta opioid receptor
- for screening cell-free protein expression using ELISA
Physical form
Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative
Legal Information
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
biological source: mouse. Quality Level: 200. conjugate: alkaline phosphatase conjugate. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: M2, monoclonal. form: buffered aqueous glycerol solution. species reactivity: all. concentration: ~1 . mg/mL. technique(s): indirect ELISA: 1:20,000. isotype: IgG1. immunogen sequence: DYKDDDDK. shipped in: wet ice. storage temp.: −. 20°C. Gene Information: human ... ALPL(249). Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A9469-.2MG
