Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Description General description Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent. F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications. Method of purification - Protein A Immunogen FLAG; peptide sequence DYKDDDDK Application Monoclonal ANTI-FLAG® M2 antibody produced in mouse has been used in: immunoblotting immunoprecipitation immunocytochemistry immunofluorescence ELISA EIA chromatin immunoprecipitation electron microscopy flow cytometry supershift assays Browse additional application references in our FLAG® Literature portal. Preparation Note: Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk Storage and Stability: Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications. Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research Legal Information: ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

1. What is the application of the ANTI-FLAG® M2 antibody? This antibody is widely used for detecting FLAG-tagged proteins via immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, ELISA, and more. 2. What is the source and type of this antibody? It is a monoclonal antibody produced in mouse and affinity-purified using Protein A. 3. Which FLAG sequences can this antibody recognize? The M2 antibody recognizes FLAG sequences at the N-terminus, Met N-terminus, C-terminus, and even internal sites. 4. How should I store the ANTI-FLAG® M2 antibody? Store at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles to maintain stability. 5. What is the recommended working concentration for applications? Dilute from 0.5–10 µg/mL in Tris Buffered Saline (pH 8.0) with 3% nonfat milk depending on the application.