General description
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Browse additional application references in our FLAG® Literature portal.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Other Notes
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Legal Information
ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
biological source: mouse. Quality Level: 200. conjugate: CY3 conjugate. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: M2, monoclonal. form: buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate). species reactivity: all. concentration: ~1 . mg/mL. technique(s): direct immunofluorescence: 10 . μ. g/mL using mammalian cells fixed with methanol:acetone. isotype: IgG1. immunogen sequence: DYKDDDDK. shipped in: dry ice. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A9594-5X1MG
