Monoclonal ANTI-FLAG(R) M2-Peroxidase (HRP) antibody produced in mouse (C15-1315-479)

General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.

Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Browse additional application references in our FLAG^® Literature portal.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

biological source: mouse. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: M2, monoclonal. form: buffered aqueous glycerol solution. species reactivity: all. concentration: ~1 . mg/mL. technique(s): indirect ELISA: 1:20,000. isotype: IgG1. immunogen sequence: DYKDDDDK. shipped in: wet ice. storage temp.: −. 20°C. Gene Information: human ... GPX1(2876). Pictograms: GHS07,GHS09. Signal Word: Warning. Hazard Statements: H315 - H317 - H319 - H411. Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.