MONOCLONAL ANTI-GLYOXALASE I ANTIBODY PRODUCED IN RAT CLONE 6F10 PURIFIED IMMUNOGLOBULIN

General description

Monoclonal Anti-Glyoxalase I (rat IgG2b isotype) is derived from the hybridoma 6F10 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a mouse Glyoxalase I fusion protein. The glyoxalase system, which consists of glyoxalase I (GLO1), glyoxalase II, and a catalytic amount of reduced glutathione (GSH), is important part of cellular metabolism. GLO1 appears to be ubiquitously expressed in all mammalian cells, suggesting its biological importance.

Immunogen

mouse Glyoxalase I fusion protein

Application

Monoclonal Anti-Glyoxalase I antibody produced in rat has been used in immunoblotting. and immunocytochemistry.

Biochem/physiol Actions

The glyoxalase system plays a major role to detoxify α-ketoaldehydes, especially methylglyoxal (MG), that are endogenously formed as a by-product of the triosephosphate isomerase reaction during glycolysis. A member of this system, GLO1 catalyzes the isomerization of a hemithioacetal, comprised of a nonenzymatic adduct of MG and glutathione (GSH), to the corresponding α-d-hydroxyacid thioester and S-D-lactoylglutathione. Studies have suggested that GLO1 may be important for brain function since, GLO1 in the brain is involved in Alzheimer′s disease, autism, anxiety and the regulation of theta oscillations during sleep.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

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