Monoclonal Anti-HA-Agarose antibody produced in mouse (C15-1314-935)

General description

The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide

Preparation Note

Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.

biological source: mouse. Quality Level: 200. conjugate: agarose conjugate. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: HA-7, monoclonal. form: PBS suspension. isotype: IgG1. capacity: 20-50 . nmol/mL elution capacity (HA-tagged fusion protein), 30-50 . nmol/mL binding capacity (HA-tagged fusion protein). shipped in: wet ice. storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US).