General description

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) (mouse IgG1 isotype) is derived from the HDAC8-48 hybridoma produced by the fusion of NS-1 mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human HDAC8.

Immunogen

recombinant human HDAC8

Application

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse has been used in :

enzyme linked immunosorbent assay (ELISA)
immunoblotting
immunofluorescence staining

Biochem/physiol Actions

Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.