General description

Binds human IgG; does not bind other human Igs.The antibody is specific for a determinant on the heavy chain of human IgG. This antibody will form antigen-antibody complexes in the liquid phase in the presence of 3% PEG 6000.

Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.

Application

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.

Disclaimer

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