Monoclonal Anti-Human IgG4 (pFc' specific) antibody produced in mouse

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. Human IgG4 is rarely found in the serum. It has the ability to bind FcγRI with high affinity. High levels of IgG4 in the serum can be pathogenic and may lead to Wegener′s granulomatosis.

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Monoclonal Anti-Human IgG4 (pFc′ specific) antibody is specific for an epitope on the pFc′ region of IgG4. The product does not react with IgG1, IgG2 and IgG3. This monoclonal antibody has been cited as the most widely applicable IgG4 specific reagent by the IUIS/WHO study.

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody has been used in immunoblotting.

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody can be used for immunoblotting and Indirect Immunoperoxidase Assay.

This clone has been singled out as most widely applicable by the IUIS/WHO study.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.