Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse

General description

Calpain-2 catalytic subunit is a protein encoded by the CAPN2 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins that are involved in essential cellular functions mediated by calcium. They share a homologous cysteine-protease domain and an E-F hand Ca²⁺-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).

Specificity

Epitope mapping studies indicate the epitope is between amino acids 502-699 (domain III/IV) of human m-calpain. The antibody reacts specifically with m-calpain. It does not cross-react with μ-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

Immunogen

m-calpain from bovine skeletal muscle.

Application

Monoclonal Anti-m-Calpain (Domain III/IV) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:50 and for western blotting at a dilution of 1:500.

Biochem/physiol Actions

Calpain plays an important role during cellular processes including cytoskeletal remodeling, apoptosis and survival. Calpain-2 over expression is significantly associated with platinum resistant tumours and in ovarian carcinomas may allow prognostic stratification of patients treated with surgery and platinum-based chemotherapy. Its over expression in basal-like or triple-negative disease is associated with adverse breast cancer-specific survival. m-calpain (calpain-2) is significantly associated with triple-negative and basal-like disease.

Physical form

Solution containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.