MONOCLONAL ANTI-MAP2 (2A+2B) ANTIBODY PRODUCED IN MOUSE CLONE AP-20 PURIFIED IMMUNOGLOBULIN (C005B-454543)

General description

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20 MAP2a appears, at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others.

Monoclonal Anti-MAP2 (2a + 2b) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with bovine microtubule associated protein 2 (MAP2).

Monoclonal Anti-MAP2 specificity was established by an immunoblot. The antibody localizes the high molecular weight forms of MAP2, namely MAP2a and MAP2b, but shows no reactivity with MAP2c. No crossreactivity is observed with MAP1, MAP5, tubulin, or tau (t).

Specificity

Monoclonal Anti-MAP2 antibody reacts with human, bovine, rat, mouse, Xenopus, salamander, and quail tissue or cells by immunocytochemical techniques utilizing either a fluorescent or peroxidase label, or by immunohistochemistry.

Immunogen

bovine MAP2

Application

Monoclonal Anti-MAP2 (2a+2b) antibody produced in mouse has been used in immunocytochemistry.

Mouse monoclonal clone AP-20 anti-MAP2 (2a+2b) antibody is used to tag MAP2 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of MAP2a and MAP2b in cell signaling especially in neural tissues.

Biochem/physiol Actions

Microtubule associated protein 2 (MAP2) is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton. MAP2 is degraded by a cathepsin D like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in others. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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