General description

Monoclonal Anti-Notch 1 (rat IgG2b isotype) is derived from the hybridoma ICN 5B5 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant fusion protein corresponding to a fragment of human Notch 1 fusion protein. Notch receptor 1 (Notch1) gene is located on human chromosome 9q34.

Application

Monoclonal Anti-Notch 1 antibody produced in rat has been used in immunoblotting.

Biochem/physiol Actions

In chronic obstructive pulmonary disease, notch receptor 1 (Notch1) helps to modulate endothelial apoptosis via the extracellular signal-regulated kinase (ERK) pathway. Overexpression of Notch1 helps to guard endothelial cell from apoptosis. Knockdown of Notch1 results in the enhancement of apoptotic cells. Notch1 serves as a tumor suppressor gene in mammalian epidermis and is a determinant of keratinocyte differentiation. Notch signaling is believed to participate in several human neoplasms including T-cell acute lymphoblastic leukemia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.