General description
The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Specificity
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting. Working dilution 1:2000
Also suitable for dot blot assays and ELISA
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Reconstitution
The antibody conjugate should be reconstituted with 0.5 ml deionized water.
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
biological source: mouse. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: purified from hybridoma cell culture. antibody product type: primary antibodies. clone: HIS-1, monoclonal. form: lyophilized powder. packaging: vial of 0.5 . mL. concentration: 5-11 . mg/mL. technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein. isotype: IgG2a. storage temp.: 2-8°C. target post-translational modification: unmodified. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116127
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- A7058-1VL
