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MONOCLONAL ANTI-POLYHISTIDINE HRP CONJUGATE (CLONE-HIS-1) (C005B-239245)

Catalog No.
C005B-239245
Mfr. No.
A7058-1VL
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
VI
Price: $1,942.06
List Price: $2,157.84

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET,

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General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting. Working dilution 1:2000
Also suitable for dot blot assays and ELISA

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

biological source: mouse. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: purified from hybridoma cell culture. antibody product type: primary antibodies. clone: HIS-1, monoclonal. form: lyophilized powder. packaging: vial of 0.5 . mL. concentration: 5-11 . mg/mL. technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein. isotype: IgG2a. storage temp.: 2-8°C. target post-translational modification: unmodified. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
UPC:
41116127
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
A7058-1VL

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