General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Components
1x MESA buffer contains:
- 40 mM MOPS
- 10 mM sodium acetate
- 1 mM EDTA (pH 8.3)
Reconstitution
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
- UPC:
- 41105323
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- M5755-1L