Motility Nitrate Medium, Buffered, 500G

Motility Nitrate Medium, Buffered is recommended for isolation and detection of Clostridium perfringens on the basis of motility and nitrate test. Peptone and HM peptone B supply amino acids and other complex nitrogenous substances. Medium being semisolid gel is used to demonstrate motility of the organism. Growth of motile organisms extends away from the line of inoculation along with turbidity. In the nitrate reduction test, on addition of Nitrate reagents a pink to red color develops if nitrite is present. Clostridium perfringens is positive for nitrate reduction and nonmotile. Clostridium absonum is weakly motile and shows weak or negative nitrate reduction. Application: Recommended For Isolation & Detection of Clostridium Perfringens On The Basis of Motility & Nitrate Test Appearance: Cream to yellow homogeneous free flowing powder Gelling: Semisolid, comparable with 0.3% Agar gel Color and Clarity: Light amber colored clear to slightly opalescent gel forms in tubes as butts Reaction of 1.95% w/v aqueous solution at 25°C. pH : 7.4±0.2 Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours