Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate (C15-1303-253)

General description

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG₁, Fc fragment.

This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.

Application

ELISA (see comments)

Enzyme Immunoassay (≥1:5000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Other Notes

Monospecific for human IgG₁ Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: secondary antibodies. clone: monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. manufacturer/tradename: Calbiochem^®. . storage condition: do not freeze. isotype: IgG. shipped in: wet ice. storage temp.: 2-8°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.