General description
Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.
This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.
Application
Enzyme Immunoassay (10 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤0.04%
Human IgG3: ≤0.02%
Human IgG4: 100%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: 100%
Human IgA: <0.01%
Human IgE: <0.01%
Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: secondary antibodies. clone: HP6025, monoclonal. form: liquid. does not contain: preservative. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 411492-400UG