Mouse anti-SUDV VP40 mAb (1G10), 100ug

Immunogen: Purified recombinant Sudan Ebola Virus VP40 (SEBOV VP40) produced in E.coli. Description: Protein A purified mouse monoclonal antibody reactive to SEBOV VP40. The antibody detects VP40 in virus-like particles (VLP) and recombinant SEBOV VP40. Fetal bovine serum was used in the production of the monoclonal antibody; a small amount of bovine IgG may have co-purified with the antibody upon purification with Protein A and thus may be present. Supplied: 100 µg is supplied in PBS at a concentration of 0.9 mg/mL. No preservative is added. Purification: Antibody is purified using immobilized protein A. Clonality: Monoclonal of the IgG1 isotype Relevance: The antibody can be used for detection of SEBOV VP40. Recommended Dilutions: ELISA: Assay-dependent dilution Western Blot: : Assay-dependent dilution. Internal QC demonstrates strong detection when using 1G10 mAb at a use dilution of 5 ng/mL. Storage: 2-3 weeks +4°C, -20°C long term. Cross Reactivity: No cross-reactivity was observed to recombinant Zaire ebolavirus VP40 (ZEBOV) or Musoke Marburg (MMARV) VP40, nor to ZEBOV and MMARV virus like particles. The ZEBOV virus like particle showed signal above background in the ELISA at the highest concentration of antibody tested. The antibody does not react to E.coli lysate from which the immunogen was expressed. Intended for research use only, not for human, therapeutic, or diagnostic applications.