Name: MOUSE CRISPR ACTIVATION CAPRANO LIBRARY
SKU: C005B-437610
Price: 33536.46 USD
Availability: InStock

MOUSE CRISPR ACTIVATION CAPRANO LIBRARY

Catalog No.

C005B-437610

Mfr. No.

PCRISPR002-1KT

Mfr. Name

Sigma-Aldrich

Qty/UOM

UOM

Price: $33,536.46

List Price: $37,262.74

General description The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and

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Description
Specifications

General description

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Application

Functional Genomics/Target Validation
Focused forward genetic activation screening
Validated positive and negative controls

Features and Benefits

Focus on your research, and we will generate your lentivirus screening library
Compatible with SAMHELPERV for consistent effector expression across a wide variety of cell lines dCas9-VP64-Blasticidin MS2-P65-HSF1-Hygromycin
Targeting Genes22,774 (Set A), 22,658 (Set B)
Compact Libary with gRNAs 67,187 (Set A), 66,889 (Set B)
Built-in Controls 500, unique non-targeting controls are in each of the two half-libraries (Set A and Set B)

Components

2 Subpools with a minimum concentration of 5x10⁸ VP/mL(via p24 assay)
PCRISPR002A - Mouse CRISPR Activation Caprano Library Set A
PCRISPR002B - Mouse CRISPR Activation Caprano Library Set B

Principle

The most effective CRISPRa system uses the CRISPR Synergistic Activation Mediator (SAM) complex. This platform combines VP64-dCas9 with a modified guide. In addition, the gRNA contains two aptamers that recruit additional co-activators, p65 and HSF1, enhancing target gene activation.

CRISPRa can also serve as a complementary approach to loss-of-function (LOF) genetic screens by enriching for a different set of genes responsible for a phenotype CRISPRa screens show little-to-no off-target activity and can be used to study genes that are difficult to characterize using only LOF approaches because of their high copy number.