Cell Line Origin
Mouse embryo fibroblast, Moloney Sarcoma Virus transformed
Cell Line Description
Mouse L cells carrying a polyhedrin promoter from baculovirus which contains the herpes TK gene and that of the Hepatitis-B surface antigen (HBS).
Application
Virus studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 every 15 days i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2. On resuscitation incubate at 33°C for one passage only then at 27°C.
Other Notes
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biological source: mouse skin. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90110101-1VL