MPBH(4-(4-N-maleimidophenyl)butyric acid hydrazide)

Product introduction:MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).Features of MPBH:• :Reactive groups:maleimide andhydrazide:• :Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups:• Long (17.9A), sulfhydryl-to-aldehyde crosslinker with an aromatic ring in its spacer arm (noncleavable):• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages:• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates:• Usesodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups:• Use withEDC to conjugate primary amine of hydrazide group to carboxyl groups:Product References:Crosslinker Application Guide -- search for recent literature references for this product:• :Reactive Moiety:Amine,Hydrazide,Maleimide :• :Crosslinker Type:Heterobifunctional :• :Form:Powder :• :Spacer:Medium(10 to 30 Å) :• :Labeling Method:Chemical Labeling :• :Spacer Arm Length:17.9 Å. Molecular Weight: 309.75