Cell Line Origin
Minipig kidney
Cell Line Description
Established from the kidney of a 118 day old foetal minipig. The cells are susceptible to some arboviruses and leptospiras and highly refractory to Chlamydia spp. and meningopneumonitis.
Application
Virus studies: arboviruses
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mini pig kidney. growth mode: Adherent. karyotype: 2n : 38, diploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41104132
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 87032604-1VL