Cell Line Origin
Human Caucasian foetal lung
Cell Line Description
Established from normal lung tissue of a 15 week old female Caucasian foetus. Like MRC-5 the line is susceptible to a wide range of viruses. At least 40 population doublings expected.
Application
Virus studies: rhinoviruses, production of viral vaccines
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human lung (fetal). growth mode: Adherent. karyotype: 2n : 46, diploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51162724
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 85020202-1VL
